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TB-500 / Thymosin β-4 fragment testing.
Market name covering full-length thymosin β-4 (43 residues, ≈4963 Da) and its short Ac-LKKTETQ fragment (≈890 Da).
The short answer
TB-500 / Thymosin β-4 fragment is tested by reverse-phase HPLC (UV 214 nm): chromatographic purity plus duplicate quantification in mg per vial against a qualified commercial reference standard. Identity is assessed by retention time against the same standard, with mass-spectrometry confirmation available as an add-on. €99 per vial, 48–72 h target turnaround, and the result is published in the public database.
What the analysis measures
Purity, quantity, identity
- Chromatographic purity (%). The main peak's share of total integrated area at UV 214 nm. It is a ratio of peptide-related peaks — not a claim about absolute mass content.
- Quantity (mg/vial). Measured content against a qualified commercial reference standard, run in duplicate; both values appear on the certificate next to the label claim.
- Identity. Retention time against the reference standard. That is presumptive identity, and the certificate says so; a mass-spectrometry match (+€79 add-on) confirms it conclusively.
The complete method — column, gradient, wavelength, limits — is published on the open method page.
Reference parameters
The molecule on paper
| Molecular weight | 4963 Da |
| Molecular formula | C212H350N56O78S |
| Class | Market name covering full-length thymosin β-4 (43 residues, ≈4963 Da) and its short Ac-LKKTETQ fragment (≈890 Da). |
| Also seen as | Thymosin beta-4, Tβ4, TB4 |
| Tariff category | Recovery & repair |
| Reference basis | A qualified commercial reference standard |
Parameters as recorded in PubChem, rounded. Your supplier's salt form and net-peptide content will differ from free-peptide values.
Worth knowing
Before you test TB-500 / Thymosin β-4 fragment
“TB-500” is the clearest identity trap in the whole tariff: two molecules more than 4,000 Da apart circulate under one name — full-length thymosin β-4 and the short acetylated LKKTETQ fragment. They elute completely differently, so a single injection resolves which one is in the vial. If you buy TB-500, this is the test to order before any other question about the material makes sense.
Price
€99 per vial, published
TB-500 / Thymosin β-4 fragment testing is €99 at the published founder rate — see its row in the price list. Add-ons: mass-spectrometry identity confirmation +€79 (adds 5–7 days), extended CoA data +€15, additional report +€29 (at order creation), rush +50%, additional vial of the same lot −30%.
On the certificate
What the CoA shows
- Measured quantity in duplicate, next to the label claim.
- Chromatographic purity with the full impurity table, not just the headline figure.
- The identity basis, stated: retention-time (presumptive) or mass-spectral (confirmed).
- Method reference and version, so the numbers can be interrogated.
- A public database entry — every result is published, verifiable by anyone, good or bad.
New to certificates? Read how to read a peptide CoA and how to verify one.
FAQ
TB-500 / Thymosin β-4 fragment testing questions
My TB-500 tested as the short fragment — is it fake?
It means the vial contains the acetylated LKKTETQ fragment rather than full-length thymosin β-4. Whether that counts as mislabelled depends on what the seller specified; many listings do not say. The certificate states which molecule was found, and that is the fact to take back to the supplier.
Which TB-500 form do you test against?
Both. The order form asks which form the label claims; identity is then run against the matching standard, and if the material does not match, the certificate says what it is consistent with instead.
How much does TB-500 / Thymosin β-4 fragment testing cost?
€99 per vial at the published founder rate: chromatographic purity plus duplicate quantification in mg per vial, with the result published in the public database. Mass-spectrometry identity confirmation is +€79, extended CoA data +€15, and an additional report +€29 at order creation.
How long does TB-500 / Thymosin β-4 fragment testing take?
The target turnaround is 48–72 hours from sample receipt. A rush option at +50% moves the sample to the front of the queue; the mass-spectrometry add-on adds 5–7 days.
Same category
Also tested in Recovery & repair
BPC-157 · GHK-Cu — or see the full price list.
Iberian Analytical opens in Q1 2027 — €99 per test, 48–72 h target, and every result published in a public database, mandatory and free. Nothing is sold on this site today. Leave an email and we will write once, when the laboratory starts issuing certificates.
Testing for a business? Apply for a trade account — volume tiers and a 24-month rate lock.