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Semaglutide testing and certificate of analysis.
GLP-1 receptor agonist analogue — 31 residues, Aib substitution, C18 fatty-diacid side chain.
The short answer
Semaglutide is tested by reverse-phase HPLC (UV 214 nm): chromatographic purity plus duplicate quantification in mg per vial against a Ph. Eur. / USP reference standard. Identity is assessed by retention time against the same standard, with mass-spectrometry confirmation available as an add-on. €149 per vial, 48–72 h target turnaround, and every report verifiable.
What the analysis measures
Purity, quantity, identity
- Chromatographic purity (%). The main peak's share of total integrated area at UV 214 nm. It is a ratio of peptide-related peaks — not a claim about absolute mass content.
- Quantity (mg/vial). Measured content against a Ph. Eur. / USP reference standard, run in duplicate; both values appear on the certificate next to the label claim.
- Identity. Retention time against the reference standard. That is presumptive identity, and the certificate says so; a mass-spectrometry match (+€79 add-on) confirms it conclusively.
The complete method — column, gradient, wavelength, limits — is published on the open method page.
Reference parameters
The molecule on paper
| Molecular weight | 4114 Da |
| Molecular formula | C187H291N45O59 |
| CAS number | 910463-68-2 |
| Class | GLP-1 receptor agonist analogue — 31 residues, Aib substitution, C18 fatty-diacid side chain. |
| Also seen as | Ozempic-class analogue (research-grade vials) |
| Tariff category | GLP-1 & metabolic |
| Reference basis | A Ph. Eur. / USP reference standard |
Parameters as recorded in PubChem, rounded. Your supplier's salt form and net-peptide content will differ from free-peptide values.
Worth knowing
Before you test Semaglutide
Semaglutide has a pharmacopoeial monograph, so it is tested against the strongest anchor available: a Ph. Eur. / USP reference standard. The market circulates both the free peptide and the acetate salt, with label claims typically between 2 and 15 mg. In public community datasets the most common failure mode is not identity but quantity — vials that contain real semaglutide, under- or over-filled against the label. That makes duplicate quantification the part of the report worth reading first.
Price
€149 per vial, published
Semaglutide testing is €149 at the published rate — see its row in the price list. Add-ons: mass-spectrometry identity confirmation +€79 (adds 5–7 days), extended CoA data +€15, additional report +€29 (at order creation), rush +50%, additional vial of the same lot −30%.
On the certificate
What the CoA shows
- Measured quantity in duplicate, next to the label claim.
- Chromatographic purity with the full impurity table, not just the headline figure.
- The identity basis, stated: retention-time (presumptive) or mass-spectral (confirmed).
- Method reference and version, so the numbers can be interrogated.
- A task ID and QR code, so anyone can verify the report.
New to certificates? Read how to read a peptide CoA and how to verify one.
FAQ
Semaglutide testing questions
Is grey-market semaglutide usually genuine?
Public community testing datasets from recent years show that most vials sold as semaglutide do contain semaglutide. Where they differ is quantity against label claim and impurity profile — which is exactly what an independent purity plus quantification test measures.
How much does Semaglutide testing cost?
€149 per vial at the published rate: chromatographic purity plus duplicate quantification in mg per vial, with a verifiable report. Mass-spectrometry identity confirmation is +€79, extended CoA data +€15, and an additional report +€29 at order creation.
How long does Semaglutide testing take?
The target turnaround is 48–72 hours from sample receipt. A rush option at +50% moves the sample to the front of the queue; the mass-spectrometry add-on adds 5–7 days.
Same category
Also tested in GLP-1 & metabolic
Tirzepatide · Retatrutide · Cagrilintide · Liraglutide · Survodutide — or see the full price list.
Iberian Analytical opens in Q1 2027 — from €99 per test, 48–72 h target, and every report verifiable. Nothing is sold on this site today. Leave an email and we will write once, when the laboratory starts issuing certificates.
Testing for a business? Apply for an associate account — a 24-month rate lock and priority scheduling.